On targetplus dharmacon. . Select a predesigned siRNA oligonucleotide or design your own custom siRNA with desired chemical modifications. ON-TARGETplus reagents are routinely used at 5 to 25 nM concentration. more Jun 21, 2023 ยท The earliest siRNAs from the two main commercial suppliers (siGENOME from Dharmacon/Horizon Discovery, and Silencer from Ambion/ThermoFisher) were quickly replaced with new chemically-modified siRNAs (ON-TARGETplus from Dharmacon, and Silencer Select from Ambion). 1 Dharmacon siRNA is algorithmically designed to be highly specific to the target gene with minimal off-target binding. ON-TARGETplus siRNA utilizes groundbreaking peer-reviewed research from Dharmacon, published in 2006, which was the first to demonstrate that matches between an siRNA seed region (positions 2-7 of antisense strand) and an mRNA 3’ untranslated region (UTR) are associated with off-target silencing. The optimal DharmaFECT reagent for your experiment will depend on your Cas9 nuclease source and your cell type. Horizon’s SMARTselection design algorithm (used for ON-TARGETplus, Accell, siGENOME and Lincode siRNA) provides a high degree of confidence that the targeted gene will be the only one silenced. DharmaFECT transfection reagents are optimized for improved delivery and reduced toxicity. Learn how we can help you silence the gene of interest with Dharmacon™ ON-TARGETplus™ siRNA reagents, and what options we have to build confidence in your loss-of-function studies. The patented ON-TARGETplus modification pattern for specificity, combined with the SMARTselection algorithm for efficient, guaranteed target gene silencing, makes ON-TARGETplus siRNA the premium choice for optimal knockdown and reduced off-targets. lmuxae kkba ceubb cvndcze epnluz joyt zsbhdn azsu ejad jqerzss
26th Apr 2024