more. Special lysis and incubation conditions reverse formaldehye modification of RNA. Higher yields (up to 30 µg) can be achieved using the High-Yield Supplementary Protocol. QIAprep Spin columns bind up to 20 ug from plasmid DNA up to 50 kb in length, while the QIAquick Spin columns binder up to 10 ug of DNA with a maximum fragment size of 10 kb. QIAquick Gel Extraction Kit包含离心柱、缓冲液和收集管，用于从凝胶（多至400 mg凝胶条）或酶促反应物中纯化DNA片段。. The design and unique binding chemistry of the QIAGEN Plasmid Plus spin columns allow a simple bind-wash-elute procedure based on a novel chemistry. advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into your laboratory workflow. With OEM by QIAGEN, you can enable your end-customers to start their analysis with high-quality spin columns that are often used as an integral part of most laboratories' workflow all over the world. The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. 0 Spin Columns (100) をQIAGENクラブ会員の方限定で販売いたしております。 The vacuum procedure provides simplified handling and faster sample processing. . Performance. The RNeasy Mini protocol is selected. Elution Microtubes CL and Caps. g. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. The kit is automatable on the QIAcube Connect. The column from QIAquick binds up to 10 ug of DNA with a maximum fragment size of 10kb. The kit includes rotor adapters that are preloaded with QIAamp spin columns and elution tubes, delivering greater convenience and time savings (see figure "Significant time savings"). , bind, wash and elute) enabling purification of high-quality DNA. ¥16,000. Ethanol is added to the flow-through from the AllPrep DNA spin column to provide appropriate binding conditions for RNA, and the sample is then applied to an RNeasy spin column, where total RNA binds to the membrane and contaminants are efficiently washed away. Contact the company and explain your situation and see what they say. QIAprep Spin columns bind up to 20 ug of plasmid DNA up to 50 kb in length, while the QIAquick Spin columns bind up to 10 ug of DNA with a maximum fragment size of 10 kb. Setup. High throughput The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. QIAprep Spin Miniprep Columns. An optional pH indicator allows easy The MinElute Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments of 70 bp – 4 kb from up to 400 mg gel slices. 0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml) 27104 询价 The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. While columns from the QIAprep Spin Miniprep Kit and the QIAquick PCR Purification- and Gel Extraction Kits are based on silica-gel-membrane technology, each is designed to work optimally within its own kit format. e. For simple and rapid homogenization of cell and tissue lysates. However, do keep in mind that the buffers themselves are not interchangeable, in the sense that some are shittier than others. 该产品不能用于疾病诊断、预防和治疗。. A lot of columns for a lot of kits from manufacturers like Qiagen are fairly interchangeable. (100) 28506 QIAquick Spin Columns 100 Buffer QG* 3 x 50 ml The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. £1,551. . QIAprep 2. In my lab I've mixed-and-matched columns from BioRad, Qiagen, BioBasic, and whatever else we have around with no repercussions. This procedure makes these kits the fastest large-scale prep available — 20 minutes for maxi and QIAquick Spin Handbook - QIAGEN. Sample preparation using QIAcube instruments follows the same steps as the manual procedure (i. Add 30–50 μl RNase-free water directly to the spin column membrane. Total RNA binds to the membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNAse-free water. The QIAvac 24 Plus For purification of up to 20 µg molecular biology grade plasmid DNA The RNeasy FFPE Kit is specially designed for purifying total RNA from formalin-fixed, paraffin-embedded tissue sections. Versatile QIAprep 2. Ethanol is added to provide appropriate binding conditions for RNA, and the sample is applied to an RNeasy spin column or 96-well plate. no. Universal – Purified nucleic acids are The QIAprep Spin Miniprep Kit or QIAwave Plasmid Miniprep Kit enables purification of up to 20 µg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. 通过简单快速的结合、洗涤、洗脱步骤，使用30–50 μl洗脱体积即可纯化得到70 bp–10 kb的DNA片段。. 5 mg (mega), 500 µg (maxi), 100 µg (midi), and 20 µg (mini) high-copy plasmid DNA is purified from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). com. Purification of Total RNA from Animal Cells Using Vacuum/Spin Technology The QIAGEN Plasmid Kits uses gravity-flow QIAGEN anion-exchange tips for efficient purification of plasmid DNA. In addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. For optimal results, we recommend using the QIAvac 24 Plus and QIAvac 96 together with the The QIAprep Spin Miniprep Kit is designed for isolating high-purity plasmid or cosmid DNA, offering up to 20 µg yields for various molecular biology applications like sequencing and cloning. Furthermore, ease of use is increased and Are these buffers interchangeable? Buffer RLT Plus contains detergents that are more ideal for binding of DNA to the AllPrep DNA mini spin columns. The QIAcube Connect uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into laboratory workflows. FAQ ID -311 Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable? The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. The vacuum procedure provides simplified handling and faster sample processing. 0 Spin Columns can be used in a microcentrifuge or on any vacuum manifold with luer connectors (e. 814KB. 0 Spin Columns can be used either in microcentrifuges, on vacuum manifolds, or in the The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. $2,604. 00. The QIAamp MinElute Virus Spin process can be fully The vacuum procedure provides simplified handling and faster sample processing. The sample is then applied to the RNeasy Mini spin column. QIAGEN QIAvac vacuum systems enable fast and efficient vacuum processing of up to 24 spin columns using the QIAvac 24 Plus, of 96-well plates using the QIAvac 96 and up to 6 HiSpeed Mega or Giga Columns using the QIAvac HiSpeed LS Vacuum. Simple – GeneJET kits feature trusted silica membrane technology in the form of a simple and convenient spin column for purification of DNA and RNA. For a selection of non-DSP kits rotor-adapters are supplied with the kits and pre-loaded with spin The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. 19413). Unincorporated nucleotides, salts, and other contaminants are removed and oligonucleotides (>17 nt) and DNA fragments ranging from 40 bp to 10 kb are purified using a simple and fast bind The QIAGEN Plasmid Plus Kits provide the most convenient method for large-scale plasmid preparation. The AllPrep DNA/RNA 96 Kit is well suited for high-throughput purification of genomic DNA and total RNA from up to 96 samples. For 50 high-purity plasmid minipreps: 50 QIAprep 2. In addition, the binding capacity and DNA recovery size cut-offs of the QIAprep and QIAquick Spin columns are different. The QIAquick Nucleotide Removal Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of oligonucleotides and DNA. In partnership with My Green Lab, we've also assessed the environmental impact of the AllPrep DNA/RNA Mini Kit (50) and the QIAwave DNA QIAprep Spin Miniprep Kit は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。 日本国内のお客様へ. Do not use spin columns manufactured by other suppliers with the QIAcube. 通过内置的 The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. Add to cart. The QIAcube Connect MDx is designed to automate selected QIAGEN DSP and non-DSP kits and the PAXgene Blood RNA Kit. QIAquick Spin Columns 50 250 Buffer QG* 2 x 50 ml 2 x 250 ml Buffer PE (concentrate) 2 x 10 ml 2 x 55 ml Buffer EB 15 ml 2 x 15 ml Collection Tubes (2 ml) 50 250 Loading Dye 100 µl 550 µl Quick-Start Protocol 1 1 QIAquick PCR & Gel Cleanup Kit Catalog no. Efficient – GeneJET kits have been extensively tested on a wide variety of sample types and demonstrated to deliver high yields with short protocols. The innovative QIAcube uses advanced technology to process QIAGEN 2. 0 spin Connect columns, enabling seamless integration of automated, low-throughput sample prep into your laboratory workflow. The cleared lysates are then applied to the QIAprep 2. QIAGEN spin columns, enabling seamless integration of automated low-throughput sample prep into your laboratory workflow. QIAquick PCR & Gel Cleanup Kit (100) For purification PCR fragments and gel extractions of 100 reactions (at least 80–100 gel reactions or 100 PCRs) 28506. For purification of up to 20 µg molecular biology grade plasmid DNA Perform this step to eliminate any possible carryover of Buffer RPE, or if residual flow-through remains on the outside of the RNeasy spin column after step 9. $153. QIAamp MinElute Virus Kits simplify viral DNA and RNA purification with fast spin-column and vacuum procedures. The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. For use with 96-well plates, including QIAamp 96 plates and RNeasy 96 plates. 0 spin columns or a QIAprep 96 plate where plasmid DNA adsorbs to the silica membrane. The kit provides spin columns for cleanup of enzymatic reactions. The AllPrep DNA/RNA Micro and Mini Kits can be automated on the QIAcube Connect. FAQ ID -311 Are the columns of that QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable? Product Details. 25 Products found. High-quality DNA is then washed and eluted from the MB Spin Column membrane for use in downstream applications including PCR and qPCR. The kits use starting sample volumes of up to 0. With over 80 protocols available for download free of charge, almost all QIAGEN spin-column kits can be automated. SPIN COLUMN 100/CS at Fishersci. QIAshredder. 0 Spin Columns can be used either in microcentrifuges, on vacuum manifolds, or in the Are Qiagen spin columns interchangeable? Are QIAprep and QIAquick Spin columns interchangeable? No. Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure. An optional pH indicator allows easy determination of the optimal pH for DNA The MinElute PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products 70 bp – 4 kb in size. The QIAamp DNA Mini QIAcube Kit simplifies isolation of DNA from human tissue samples with fast spin-column procedures. The lysate is then passed through a gDNA Eliminator spin column or gDNA Eliminator 96 plate that, in combination with the high-salt buffer, selectively and efficiently removes genomic DNA. Using our spin columns as an OEM component will help you ease the regulatory compliance burden while our global reach can simplify your logistics My QIAGEN; Plan your experiment; Discovery & Translational Research; Diagnostics & Clinical Research; Are QIAprep and QIAquick Spin columns interchangeable? No. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An optional pH indicator allows easy determination of the optimal pH for DNA The vacuum procedure provides simplified handling and faster sample processing. No. For purification of up to 20 μg molecular biology grade plasmid DNA. High-throughput options: We offer a vacuum-based protocol for faster processing without centrifugation for the DNA-binding and columnwashing steps using MB Spin Columns. 0 Spin Columns can be used either in microcentrifuges, on vacuum manifolds, or in the The column is washed and pure, ready-to-use DNA is then eluted. Knowledgeable and professional Product & Technical Support. QIAquick Gel Extraction Kit (250) For gel extraction or cleanup of 250 reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) 28706. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube Connect (see figure "QIAcube Connect"). English. 24/7 automatic processing of online orders. Versatile QIAprep spin columns can be used either in microcentrifuges, on vacuum manifolds, or in the QIAcube (see figures "QIAprep Spin Yes they are interchangeable. We would like to show you a description here but the site won’t allow us. To work even faster, get kits specifically designed for use with QIAcube Connect. Sample preparation using the QIAcube follows the same steps as the manual procedure (i. QIAquick Gel Extraction Kit (1000) For gel extraction or cleanup of 1000 reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) 28706X4. The spin columns are designed to allow elution in very small volumes (as little as 10 µL), delivering high yields of highly concentrated DNA. , lyse, bind, wash, and elute), enabling you to continue using the miRNeasy Micro Kit for purification of high-quality miRNA For purification of up to 20 µg molecular biology grade plasmid DNA an MB Spin Column. QIAGENクラブ会員限定製品のご案内： QIAprep 2. This virtual prep demonstrates RNA purification using the RNeasy Mini Kit. File size. Spot an opportunity for improvement? Shop Qiagen, Inc. These come with spin columns and tubes already loaded onto the rotor, so you don't have to put them in yourself: The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. , QIAvac 24 Plus, cat. Optimized high-yield protocols and extra buffer volumes are provided with the kit, enabling yields from 250 μg (Midi) to 10 mg (Giga). The QIAprep Spin Miniprep Kit is designed for isolating high-purity plasmid or cosmid DNA, offering up to 20 µg yields for various molecular biology applications like sequencing and cloning. Catalog No. Fast and reliable (re)-ordering. Using a microcentrifuge or vacuum manifold, high concentration of DNA fragment (70 bp The vacuum procedure provides simplified handling and faster sample processing. ¥66,000. not sure about the DNeasy column, I assume it will bind up to 20 ug of plasmid DNA up to 50 kb in length US$128. 11. 0 Spin Columns can be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. QIAprep Spin Miniprep Columns 旨在用于分子生物学应用。. Log in To see your account pricing. Sample preparation using the QIAcube Connect follows the same steps 28704. NC0232632. Place the RNeasy spin column in a new 1. The MinElute Reaction Cleanup Kit ensures cleanup of up to 5 µg DNA (70 bp to 4 kb) from enzymatic reactions, delivering high yields of DNA suitable for a range of applications. The RNA application is selected. Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable? Yes - all QIAquick Spin Kits contain identical columns, but different binding buffers optimized for each specific application. Quantity. The spin columns fit into a conventional table-top microcentrifuge. The QIAprep Spin Miniprep Kit or QIAwave Plasmid Miniprep Kit enables purification of up to 20 µg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Add to Cart. But RLT Plus is not compatible with Buffer APP used to precipitate proteins in AllPrep DNA/RNA/Protein Mini kit. QIAquick Spin Handbook - (EN) Print Bookmark Share. An optional pH indicator allows easy determination of the optimal pH for DNA Product Details. Kits are currently available for purification of RNA, genomic DNA and viral RNA. 60 / Case of 100. First, bacterial cultures are lysed, and the lysates are cleared by centrifugation or filtration. 0 Spin Columns can be used either in microcentrifuges, on vacuum manifolds, or in the 包含凝胶上样染料，方便样本分析. QIAcube Connect lets you automate your manual spin-column-based sample preparation kits right out of the box and purify up to 12 samples in a single run. The resulting highly concentrated DNA is ready for immediate The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. Watch the video to see how the QIAcube takes you through the process step-by The vacuum procedure provides simplified handling and faster sample processing. The QIAcube is designed for fully automated processing of up to 12 samples using QIAGEN spin-column kits. 5 ml collection tube (supplied). The QIAquick Nucleotide Removal Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube Connect, enabling increased productivity and standardization of results (see figures "Spin column handling options A " and " QIAcube Connect "). Up to 10 mg (giga), 2. Damage caused by use of other types of spin columns will void the instrument warranty. The QIAprep Spin Miniprep Kit is designed for quick and convenient processing of 1–24 samples simultaneously in less than 30 minutes. The design and unique binding chemistry of the QIAGEN Plasmid Plus columns allow a simple bind-wash-elute procedure based on a novel chemistry. ll bind, wash, and elution steps are perfA ormed by centrifugation in a microcentrifuge. QIAGEN kits for IVD and for research applications. Format. The QIAprep Spin Miniprep Kit can be fully automated on the QIAcube ® Connect. 2 ml and combine the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl. Due to product restrictions, please Sign In to purchase or view availability for this product. All steps in the purification procedure are fully automated — and up to 12 samples can be processed per run. pdf. lnvhrclwixbjpbvbdqsx